EPCR Antibody [Unconjugated]

Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EPCR at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EPCR was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse EPCR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2749) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse EPCR Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2749, filled histogram) or control antibody (Catalog # AB‑108‑C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

• Reactivity: Mu
• Applications: WB, Flow, IP, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EPCR Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EPCR
  Ala17-Ser214
  Accession # Q64695
• Specificity: Detects mouse EPCR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human EPCR is observed.
• Details of Functionality: ActivityAssaywCitation not found None None None None
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PROCR

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunoprecipitation 25 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EPCR Antibody [Unconjugated]

• APC receptor
• CCCA
• CCD41
• CCD41centrosome-associated protein
• CD201 antigen
• CD201
• centrocyclin
• Endothelial cell protein C receptor
• endothelial protein C receptor
• EPCR
• EPCRMGC23024
• PROCR
• protein C receptor, endothelial

Background

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EPCR Antibody (AF2749)(6)

Publications using AF2749

• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC, Mouse)
• Greineder, Colin F, Chacko, Ann-Mari, Zaytsev, Sergei, Zern, Blaine J, Carnemolla, Ronald, Hood, Elizabet, Han, Jingyan, Ding, Bi-Sen, Esmon, Charles, Muzykantov, Vladimir Vascular immunotargeting to endothelial determinant ICAM-1 enables optimal partnering of recombinant scFv-thrombomodulin fusion with endogenous cofactor. PLoS ONE, 2013;8(11):e80110. 2013 [PMID: 24244621] (ICC, Mouse)
• N-cadherin+ HSCs in fetal liver exhibit higher long-term bone marrow reconstitution activity than N-cadherin- HSCs. Biochem Biophys Res Commun, 2012;428(3):354-9. 2012 [PMID: 23092738] (Flow Cytometry, Mouse)
• Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice. J. Biol. Chem., 2012;287(20):16356-64. 2012 [PMID: 22447930] (IHC-P, Mouse)
• Disse J, Petersen HH, Larsen KS The Endothelial Protein C Receptor Supports Tissue Factor Ternary Coagulation Initiation Complex Signaling through Protease-activated Receptors. J. Biol. Chem., 2011;286(7):5756-67. 2011 [PMID: 21149441] (Western Blot, Mouse)
• Finigan JH, Boueiz A, Wilkinson E, Damico R, Skirball J, Pae HH, Damarla M, Hasan E, Pearse DB, Reddy SP, Grigoryev DN, Cheadle C, Esmon CT, Garcia JG, Hassoun PM Activated protein C protects against ventilator-induced pulmonary capillary leak. Am. J. Physiol. Lung Cell Mol. Physiol., 2009;296(6):L1002-11. 2009 [PMID: 19363121] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PROCR
• Uniprot:
  • Q64695()