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EPCR Antibody (432714) [Unconjugated]

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bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse EPCR Monoclonal Antibody (Catalog # MAB2749, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse EPCR Monoclonal Antibody (Catalog # MAB2749, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
432714
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2749U-100UG
Alexa Fluor 405
FAB2749V-100UG
Alexa Fluor 488
FAB2749G-100UG
Alexa Fluor 594
FAB2749T-100UG
Alexa Fluor 647
FAB2749R-100UG
Alexa Fluor 700
FAB2749N-100UG
Alexa Fluor 750
FAB2749S-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB2749-SP
Carrier Free
FAB2749V-100UG
Carrier Free
FAB2749U-100UG
Carrier Free
FAB2749T-100UG
Carrier Free
FAB2749S-100UG
Carrier Free
FAB2749R-100UG
Carrier Free
FAB2749N-100UG
Carrier Free
FAB2749G-100UG
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EPCR Antibody (432714) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EPCR
Ala17-Ser214
Accession # Q64695
Specificity
Detects mouse EPCR in direct ELISAs.
Details of Functionality
ActivityAssaywCitation not found None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
PROCR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EPCR Antibody (432714) [Unconjugated]

  • APC receptor
  • CCCA
  • CCD41
  • CCD41centrosome-associated protein
  • CD201 antigen
  • CD201
  • centrocyclin
  • Endothelial cell protein C receptor
  • endothelial protein C receptor
  • EPCR
  • EPCRMGC23024
  • PROCR
  • protein C receptor, endothelial

Background

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Thrombomodulin/BDCA-3 ...
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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB2749U
Alexa Fluor 405 FAB2749V
Alexa Fluor 488 FAB2749G
Alexa Fluor 594 FAB2749T
Alexa Fluor 647 FAB2749R
Alexa Fluor 700 FAB2749N
Alexa Fluor 750 FAB2749S
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol PROCR
Uniprot