ENPP-2/Autotaxin Antibody

Immunocytochemistry/Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Autotaxin protein stained by Autotaxin antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Western Blot: ENPP-2/Autotaxin Antibody [NBP1-32162] - Human Cerebrospinal Fluid (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ENPP-2/Autotaxin antibody (NBP1-32162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Bv
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ENPP-2/Autotaxin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ENPP-2/Autotaxin. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENPP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry-Paraffin Reported in scientific literature (PMID: 28739650)
  • Western Blot 1:500-1:3000
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ENPP-2/Autotaxin Antibody

• ATX
• ATXFLJ26803
• ATX-X
• Autotaxin
• autotaxin-t
• EC 3.1.4.39
• ectonucleotide pyrophosphatase/phosphodiesterase 2
• ectonucleotide pyrophosphatase/phosphodiesterase family member 2
• E-NPP 2
• ENPP2
• ENPP-2
• Extracellular lysophospholipase D
• Lysophosphatidic Acid
• LysoPLD
• NPP2
• PD-IALPHA
• PDNP2
• PDNP2NPP2
• phosphodiesterase I/nucleotide pyrophosphatase 2
• plasma lysophospholipase D

Background

The protein encoded by the ENPP2 gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Publications for ENPP-2/Autotaxin Antibody (NBP1-32162)(1)

Publication using NBP1-32162

• Zhang MZ, Wang X, Yang H et al. Lysophosphatidic Acid Receptor Antagonism Protects against Diabetic Nephropathy in a Type 2 Diabetic Model J Am Soc Nephrol. 2017-07-23 [PMID: 28739650] (IHC-P, WB, Mouse)

Bioinformatics

• Gene Symbol: ENPP2
• Entrez:
  • 5168(Human)
  • 18606 (Mouse)
• Uniprot:
  • Q13822()