Recombinant Human Enolase 2/Neuron-specific Enolase Protein

Images

 
SDS-Page: Recombinant Human Enolase 2/Neuron-specific Enolase Protein [NBC1-18342] - [NBC1-18342] - 47 kDa ( 434 aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

Order Details

Recombinant Human Enolase 2/Neuron-specific Enolase Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-434 of Human Enolase 2/Neuron-specific Enolase

Source: E.coli

Amino Acid Sequence: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL

Details of Functionality
Specific activity is > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
ENO2
Purity
>95% pure by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBC1-18342 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 7.5), 0.1 M KCl, 5mM MgSO4
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human Enolase 2/Neuron-specific Enolase Protein

  • 2-phospho-D-glycerate hydrolyase
  • 2-phospho-D-glycerate hydro-lyase
  • EC 4.2.1.11
  • ENO2
  • enolase 2 (gamma, neuronal)
  • Enolase 2
  • gamma-Enolase
  • Neural enolase
  • neuron specific gamma enolase
  • neurone-specific enolase
  • Neuronspecific Enolase
  • Neuron-specific enolase
  • NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00002023-M01
Species: Hu, Fe
Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IHC-P, IP
NB300-141
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-653
Species: Hu, Mu, Rt, Ch, Fe, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB120-15160
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45267
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34594
Species: Hu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
AF4478
Species: Hu
Applications: WB, IHC
AF3844
Species: Hu, Mu
Applications: IHC
7954-GM/CF
Species: Hu
NBP2-42388
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NB120-14817
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, S-ELISA
NBP1-87103
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-60046
Species: Hu, Mu, Rt, Po, Bv, Eq, Gt, Gp, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-37447
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, IF
NBP1-05163
Species: Hu, Rt, Po, Bv
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB600-717
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Gp, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NBC1-18342
Species: Hu
Applications: Func, PAGE, Bioactivity

Publications for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)(2)

We have publications tested in 1 confirmed species: Monkey.

We have publications tested in 2 applications: ELISA, WB.


Filter By Application
ELISA
(1)
WB
(1)
All Applications
Filter By Species
Monkey
(1)
All Species

Reviews for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342) (0)

There are no reviews for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

View specific protocols for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Enolase 2/Neuron-specific Enolase Products

Bioinformatics Tool for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)

Discover related pathways, diseases and genes to Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)

Discover more about diseases related to Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342).
 

Pathways for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)

View related products by pathway.

PTMs for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)

Learn more about PTMs related to Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342).
 

Research Areas for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)

Find related products by research area.

Blogs on Enolase 2/Neuron-specific Enolase

There are no specific blogs for Enolase 2/Neuron-specific Enolase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

GFAP Antibody
NB300-141

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Enolase 2/Neuron-specific Enolase Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol ENO2