Recombinant Human Enolase 2/Neuron-specific Enolase Protein

SDS-Page: Recombinant Human Enolase 2/Neuron-specific Enolase Protein [NBC1-18342] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Discontinued

Recombinant Human Enolase 2/Neuron-specific Enolase Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-434 of Human Enolase 2/Neuron-specific Enolase
  
  

  Source: E.coli

  Amino Acid Sequence: MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL

• Details of Functionality: Specific activity is > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ENO2
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 47.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 7.5), 0.1 M KCl, 5mM MgSO4
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Enolase 2/Neuron-specific Enolase Protein

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Enolase 2/Neuron-specific Enolase Recombinant Protein (NBC1-18342)(2)

Publications using NBC1-18342

• Ramirez-Celis A, Becker M, Nuño M, et al. Risk assessment analysis for maternal autoantibody-related autism (MAR-ASD): a subtype of autism Molecular psychiatry 2021-01-22 [PMID: 33483694]
• Ramirez-Celis A, Edmiston E, Schauer J, et al. Peptides of neuron specific enolase as potential ASD biomarkers: From discovery to epitope mapping Brain Behav. Immun. 2019-12-05 [PMID: 31812776] (WB, ELISA, Monkey)

Bioinformatics

• Gene Symbol: ENO2