Human Enolase 2/Neuron-specific Enolase ELISA Kit (Colorimetric) Summary
| Description |
ENO2 (Human) ELISA Kit is an enzyme immunoassay for the quantitative measurement of ENO2 in serum. |
| Sensitivity |
0.19 ng/mL |
| Assay Type |
Quantification |
| Inter-Assay |
CV% <11.2%. |
| Intra-Assay |
CV% < 4.4%. |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
ENO2 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Conjugate: Monoclonal anti hNSE antibody conjugated with horseradish peroxidase (HRP).
- Control 1 (lyophilized): Negative control, reconstitute with 0.75 mL deionized H2O before use.
- Control 2 (lyophilized): Positive control, reconstitute with 0.75 mL deionized H2O before use.
- Incubation buffer: Phosphate buffer (50 mM), pH 7.4; BSA (1 g/L).
- Microplate: Breakable microplate, monoclonal anti hNSE antibody adsorbed on themicroplate.
- Note: The Standards and Controls contain hNSE in a proteic stabilizing matrix solution.
- Standard A (Standard 0) (lyophilized): Reconstitute with 0.75 mL deionized H2O before use.
- Standard B (Standard 1) (lyophilized): Reconstitute with 0.75 mL deionized H2O before use.
- Standard C (Standard 2) (lyophilized): Reconstitute with 0.75 mL deionized H2O before use.
- Standard D (Standard 3) (lyophilized): Reconstitute with 0.75 mL deionized H2O before use.
- Standard E (Standard 4) (lyophilized): Reconstitute with 0.75 mL deionized H2O before use.
- Stop Solution: Sulphuric acid (0.15 mol/L) (avoid any skin contact).
- TMB Substrate: H2O2-TMB (0.26 g/L) (avoid any skin contact).
- Wash Solution: 50x concentrate, NaCl (45 g/L); Tween 20 (55 g/L).
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human Enolase 2/Neuron-specific Enolase ELISA Kit (Colorimetric)
Background
Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for Enolase 2/Neuron-specific Enolase ELISA Kit (KA1910). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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