Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Western blot showing a single band at the correct MW (47 kDa).

Immunocytochemistry/Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Dissociated cell cultures of neonatal mouse brains, showing NSE-2 (green staining) in neurons. These cultures were counter-stained with a rabbit anti-GFAP to localize astrocytes, as well as with DAPI (blue staining) to localize nuclei.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Enolase 2/Neuron-specific Enolase Antibody Summary

• Immunogen: Chickens were immunized with two synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Enolase 2/Neuron-specific Enolase gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences.
• Marker: Neuron Cell Marker
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: ENO2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:10000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10000-1:20000
• Application Notes: Both of these anti-peptide antibodies were analyzed by immunohistochemistry (1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500) as the secondary reagent.
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

NB 100-1606 reacts with mouse NSE type 2. The peptide sequences are shared between the human and rat sequences.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Sodium PBS (0.9% isotonic, w/v, pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Notes

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2
• Entrez:
  • 2026(Human)
  • 13807(Mouse)
  • 24334(Rat)
• Uniprot:
  • P09104()