Enolase 2/Neuron-specific Enolase Antibody (5G10)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-Fr, IHC-P, S-ELISA
Clone
5G10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Enolase 2/Neuron-specific Enolase Antibody (5G10) Summary

Immunogen
Purified Neuron Specific Enolase from human brain
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
Reacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
ENO2
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
Application Notes
Recommended pairs for sandwich immunoassay (capture-detection): NB120-8324 - NB200-422
Positive Control
Enolase 2/Neuron-specific Enolase Lysate (NBL1-10268)

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (5G10)

  • 2-phospho-D-glycerate hydrolyase
  • 2-phospho-D-glycerate hydro-lyase
  • EC 4.2.1.11
  • ENO2
  • enolase 2 (gamma, neuronal)
  • Enolase 2
  • gamma-Enolase
  • Neural enolase
  • neuron specific gamma enolase
  • neurone-specific enolase
  • Neuronspecific Enolase
  • Neuron-specific enolase
  • NSE

Background

Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ENO2
Entrez