Enolase 2/Neuron-specific Enolase Antibody (5A4)

Product Discontinued

Enolase 2/Neuron-specific Enolase Antibody (5A4) Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified NSE from human brain.
• Localization: Cytoplasmic
• Marker: Neuronal Marker
• Specificity: Human NSE (5A4).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Reacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts. Recommended pairs for sandwich immunoassay (coating - conjugate): NB120-10169 - NB200-422.
• Control:

  Enolase 2/Neuron-specific Enolase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (5A4)

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2
• Entrez:
  • 2026(Human)