Enolase 2/Neuron-specific Enolase Antibody (1A3)

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (1A3) [H00002026-M01] - Analysis of ENO2 expression in transfected 293T cell line by ENO2 monoclonal antibody (M01), clone 1A3.Lane 1: ENO2 transfected lysate(47.3 KDa).Lane 2: Non-transfected lysate.

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (1A3) [H00002026-M01] - ENO2 monoclonal antibody (M01), clone 1A3. Analysis of ENO2 expression in different cell lines.

ELISA: Enolase 2/Neuron-specific Enolase Antibody (1A3) [H00002026-M01] - Detection limit for recombinant GST tagged ENO2 is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

Enolase 2/Neuron-specific Enolase Antibody (1A3) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ENO2 (AAH02745, 325 a.a. ~ 434 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PKRIERAVEEKACNCLLLKVNQIGSVTEAIQACKLAQENGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEELGDEARFAGHNFRNPSVL
• Localization: Cytoplasmic.
• Marker: Neuronal Marker
• Specificity: ENO2 - enolase 2 (gamma, neuronal)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (1A3)

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enolase 2/Neuron-specific Enolase Antibody (H00002026-M01)(1)

Publication using H00002026-M01

• Chang GC, Liu KJ, Hsieh CL et al. Identification of alpha-Enolase as an Autoantigen in Lung Cancer: Its Overexpression Is Associated with Clinical Outcomes. Clin Cancer Res;12(19):5746-54. 2006-10-01 [PMID: 17020980]

Bioinformatics

• Gene Symbol: ENO2
• Entrez:
  • 2026(Human)
• OMIM:
  • 131360(Human)
• Uniprot:
  • AAH02745(Human)