Enolase 1 Antibody (JM63-53)

Images

 
Western Blot: Enolase 1 Antibody (JM63-53) [NBP2-75481] - Western blot analysis of Enolase 1 on Zebrafish tissue lysates using anti-Enolase 1 antibody at 1/1,000 dilution.
Flow Cytometry: Enolase 1 Antibody (JM63-53) [NBP2-75481] - Analysis of MCF-7 cells with ENO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western Blot: Enolase 1 Antibody (JM63-53) [NBP2-75481] - Analysis of ENO1 on different lysates using anti-ENO1 antibody at 1/1,000 dilution.Positive control:Lane 1: Rat brain tissue Lane 2: NIH-3T3Lane 3: Hela ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
JM63-53
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Enolase 1 Antibody (JM63-53) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Recombinant protein (SwissProt: P06733 Human;SwissProt: P17182 Mouse;SwissProt: P04764 Rat)
Localization
Nucleus and Cytoplasm. Cell membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ENO1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:10-1:50
  • Western Blot 1:500-1:2000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Enolase 1 Antibody (JM63-53)

  • 2-phospho-D-glycerate hydro-lyase
  • Alpha enolase
  • C-myc promoter-binding protein
  • c-myc promoter-binding protein-1
  • EC 4.2.1
  • EC 4.2.1.11
  • ENO1L1
  • Enolase 1
  • enolase 1, (alpha)
  • MBP-1
  • MBPB1
  • MPB-1
  • MPB1c-myc promoter-binding protein 1
  • MYC promoter-binding protein 1
  • NNE
  • Non-neural enolase
  • Phosphopyruvate hydratase
  • Plasminogen-binding protein
  • PPHalpha enolase like 1
  • tau-crystallin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ENO1