Enolase 1 Antibody (1G1)

Western Blot: Enolase 1 Antibody (1G1) [H00002023-M04] - Analysis of ENO1 expression in human colon.

Western Blot: Enolase 1 Antibody (1G1) [H00002023-M04] - Analysis of ENO1 expression in Raw 264.7 (Cat # L024V1).

Western Blot: Enolase 1 Antibody (1G1) [H00002023-M04] - Analysis of ENO1 expression in MCF-7.

Western Blot: Enolase 1 Antibody (1G1) [H00002023-M04] - Analysis of ENO1 expression in HeLa (Cat # L013V1).

Product Discontinued

Enolase 1 Antibody (1G1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
• Specificity: ENO1 (1G1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Enolase 1 Antibody (1G1)

• 2-phospho-D-glycerate hydro-lyase
• Alpha enolase
• C-myc promoter-binding protein
• c-myc promoter-binding protein-1
• EC 4.2.1
• EC 4.2.1.11
• ENO1L1
• Enolase 1
• enolase 1, (alpha)
• MBP-1
• MBPB1
• MPB-1
• MPB1c-myc promoter-binding protein 1
• MYC promoter-binding protein 1
• NNE
• Non-neural enolase
• Phosphopyruvate hydratase
• Plasminogen-binding protein
• PPHalpha enolase like 1
• tau-crystallin

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enolase 1 Antibody (H00002023-M04)(1)

Publication using H00002023-M04

• Blandin G, Marchand S, Charton K et al. A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome. Skelet Muscle 2013-02-15 [PMID: 23414517] (WB, Human)

Bioinformatics

• Gene Symbol: ENO1
• Entrez:
  • 2023(Human)
• OMIM:
  • 172430(Human)
• Uniprot:
  • AAH22545(Human)