ENO3 Antibody - BSA Free

Western Blot: ENO3 Antibody [NBP1-31764] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Molt-4 C: Raji 10% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-31764] - Paraffin-embedded gastric carcinoma; antibody dilution 1:500.

Western Blot: ENO3 Antibody [NBP1-31764] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.

Western Blot: ENO3 Antibody [NBP1-31764] - 30 ug Rat 2 whole cell lysate/extract 10% SDS-PAGE gel, antibody dilution 1:1000.

Immunohistochemistry: ENO3 Antibody [NBP1-31764] - Immunohistochemical analysis (whole mount) of zebrafish embryo, using ENO3 antibody (NBP1-31764) at 1:200 dilution.

Western Blot: ENO3 Antibody [NBP1-31764] - Sample (30 ug of whole cell lysate) A: Zebrafish muscle 10% SDS PAGE NBP1-31764 diluted at 1:1000

• Reactivity: Hu, Mu, Rt, Ze, Po, Bv, Rb, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ENO3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ENO3 Antibody - BSA Free (NBP1-31764) is a polyclonal antibody validated for use in IHC and WB. Anti-ENO3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ENO3. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (98%), Bovine (98%), Rabbit (98%), Xenopus (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENO3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%), Zebrafish (89%), Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ENO3 Antibody - BSA Free

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• beta-enolase
• EC 4.2.1
• EC 4.2.1.11
• enolase 3 (beta, muscle)
• Enolase 3
• enolase 3, (beta, muscle)
• GSD13
• MSE
• Muscle-specific enolase
• Skeletal muscle enolase

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ENO3 Antibody (NBP1-31764)(1)

Publication using NBP1-31764

• Hadrevi J, Hellstrom F, Kieselbach T et al. Protein differences between human trapezius and vastus lateralis muscles determined with a proteomic approach. BMC Musculoskelet Disord 2011-08-01 [PMID: 21831281] (WB, Human)

Bioinformatics

• Gene Symbol: ENO3