ENO3 Antibody (S07-5B2)

Western Blot: ENO3 Antibody (S07-5B2) [NBP3-14890] - Western blot detection of ENO3 in Rat Brain, C6, 3T3, Hela cell lysates using ENO3 Rabbit mAb (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 47kDa.

Immunocytochemistry/Immunofluorescence: ENO3 Antibody (S07-5B2) [NBP3-14890] - Immunofluorescence of ENO3 (green) in Hela cells using NBP3-14890 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S07-5B2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

ENO3 Antibody (S07-5B2) Summary

• Description: Novus Biologicals Rabbit ENO3 Antibody (S07-5B2) (NBP3-14890) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human ENO3 (Uniprot#: P13929)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ENO3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for ENO3 Antibody (S07-5B2)

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• beta-enolase
• EC 4.2.1
• EC 4.2.1.11
• enolase 3 (beta, muscle)
• Enolase 3
• enolase 3, (beta, muscle)
• GSD13
• MSE
• Muscle-specific enolase
• Skeletal muscle enolase

Background

ENO3 encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO3