Enah/Vasp-like Antibody

Western Blot: Enah/Vasp-like Antibody [NBP1-36954] - (1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Enah/Vasp-like Antibody Summary

• Immunogen: Peptide with sequence C-SQPPNSSEAGRKP corresponding to internal region according to NP_057421.1.
• Epitope: internal region
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EVL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in Jurkat lysates (calculated MW of 44.8 kDa band according to NP_057421.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Enah/Vasp-like Antibody

• ena/VASP-like protein
• Enah/Vasp-like
• RNB6Ena/vasodilator-stimulated phosphoprotein-like

Background

Enah/Vasp-like (EVL) is a member of the Ena/VASP protein family. Ena/VASP proteins are actin-associated proteins involved in a range of processes that require dynamic actin remodeling such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. EVL enhances actin nucleation and polymerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enah/Vasp-like Antibody (NBP1-36954)(1)

Publication using NBP1-36954

• Dent EW, Kwiatkowski AV, Mebane LM et al. Filopodia are required for cortical neurite initiation. Nat Cell Biol 2007-12-01 [PMID: 18026093]

Bioinformatics

• Gene Symbol: EVL
• Entrez:
  • 51466(Human)
  • 14026(Mouse)
  • 79115(Rat)
• Uniprot:
  • NP_057421.1(Human)