EMP2 Overexpression Lysate (Native)

Images

 
Western Blot: EMP2 Overexpression Lysate (Native) [NBL1-10260] - analysis of EMP2 in EMP2 Overexpression Lysate using anti-EMP2 antibody (Cat.# NBP1-86847, 1:500 dilution). Image from verified customer review.
Western Blot: EMP2 Overexpression Lysate (Adult Normal) [NBL1-10260] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EMP2.

Product Details

Summary
Applications WB

Order Details

EMP2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201995 and based on accession number NM_001424. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens epithelial membrane protein 2 (EMP2), mRNA.
Gene
EMP2

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
RIPA buffer

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBL1-10260 in the following application:

Lysate Details for EMP2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EMP2 Overexpression Lysate (Native)

  • EMP2
  • epithelial membrane protein 2
  • MGC9056
  • Protein XMP
  • XMP
  • XMPEMP-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-91705
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83107
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00008833-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-60837
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-67670
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
H00002012-P01
Species: Hu
MAB4505
Species: Hu
Applications: ICFlow
NBP1-59438
Species: Hu
Applications: WB, ELISA, IHC
NBP1-80656
Species: Hu
Applications: IHC, IHC-P
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MAB6240
Species: Hu
Applications: WB, IHC
MAB7288
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP1-37775
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP2-45897
Species: Hu
Applications: WB, IHC
NB100-615
Species: Hu, Mu, Rt, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-89192
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-10260
Applications: WB

Publications for EMP2 Lysate (NBL1-10260) (0)

There are no publications for EMP2 Lysate (NBL1-10260).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EMP2 Lysate (NBL1-10260) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NBL1-10260:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot EMP2 NBL1-10260
Enlarge
reviewed by:
Anonymous
WB Mouse 01/26/2015
View

Summary

ApplicationWestern Blot
Sample TestedEMP2 overexpression lysate
SpeciesMouse
Lot2F2A46

Blocking

Blocking Details2% cold fish geletin in TBS buffer, room temperature for 1hour

Primary Anitbody

Dilution RatioEMP2 antibody Novus NB1-86847, Rabbit 1:500.

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signaling, #7074
Secondary Concentration1:5000

Details

Detection Notes1 min exposure shows band at correct MW.

Comments

CommentsThis EMP2 overexpression lysates worked very well in my EMP2 blot. When loaded equal amount, the EMP2 lysates have strong bands compare with almost no any bands in vector.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EMP2 Lysate (NBL1-10260) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EMP2 Overexpression Lysate (Native) Products

Related Products by Gene

Bioinformatics Tool for EMP2 Lysate (NBL1-10260)

Discover related pathways, diseases and genes to EMP2 Lysate (NBL1-10260). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EMP2 Lysate (NBL1-10260)

Discover more about diseases related to EMP2 Lysate (NBL1-10260).
 

Pathways for EMP2 Lysate (NBL1-10260)

View related products by pathway.

PTMs for EMP2 Lysate (NBL1-10260)

Learn more about PTMs related to EMP2 Lysate (NBL1-10260).

Blogs on EMP2

There are no specific blogs for EMP2, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol EMP2
Entrez

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-10260 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

EMP2 Recombinant Protein
H00002013-P01-10ug
EMP2 Recombinant Protein
H00002013-P01-10ug
EMP2 Recombinant Protein
H00002013-P01-10ug
EMP2 Antibody
NBP1-86847
EMP2 Antibody
NBP1-86847
EMP2 Antibody
NBP1-86847