P2X7/P2RX7 Antibody

Western Blot: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of paraffin embedded Human Kidney. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-37775] - Staining of paraffin embedded Human Brain Cortex. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

P2X7/P2RX7 Antibody Summary

• Immunogen: Peptide with sequence YETNKVTRIQSMNY-C corresponding to N-Terminus according to NP_002553.2.
• Epitope: N-Terminus
• Predicted Species: Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: P2RX7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA detection limit 1:16000
  • Western Blot 1 - 3 ug/mL
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:34391810)

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:34391810).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for P2X7/P2RX7 Antibody

• ATP receptor
• MGC20089
• P2RX7
• P2X purinoceptor 7
• P2X7
• P2X7P2X7 receptor
• P2X7R
• P2Z receptor
• purinergic receptor P2X, ligand-gated ion channel, 7
• purinergic receptor P2X7 variant A
• Purinergic receptor

Background

The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants which would encode different isoforms have been identified although some fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2X7/P2RX7 Antibody (NBP1-37775)(4)

Publications using NBP1-37775

• Alnafisah R, Lundh A, Asah SM et al. Altered purinergic receptor expression in the frontal cortex in schizophrenia Schizophrenia (Heidelberg, Germany) 2022-11-14 [PMID: 36376358] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Carvalho K, Martin E, Ces A Et al. P2X7-deficiency improves plasticity and cognitive abilities in a mouse model of Tauopathy Progress in neurobiology 2021-08-12 [PMID: 34391810] (ICC/IF, IF/IHC, Mouse)
• Martin E, Amar M, Dalle C, et al New role of P2X7 receptor in an Alzheimer's disease mouse model. Mol Psychiatry 2018-06-22 [PMID: 29934546] (IHC-P, Human)
• Tomasinsig L, Pizzirani C, Skerlavaj B et al. The human cathelicidin LL-37 modulates the activities of the P2X7 receptor in a structure-dependent manner. J Biol Chem 2008-11-01 [PMID: 18765670]

Bioinformatics

• Gene Symbol: P2RX7
• Entrez:
  • 5027(Human)
  • 18439(Mouse)
  • 29665(Rat)
• Uniprot:
  • NP_002553.2(Human)