Emerin Antibody (CL0203) - BSA Free

Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Analysis of extracts from RT-4 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-EMD monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U2OS cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human testis shows nuclear membrane immunoreactivity.

Western Blot: Emerin Antibody (CL0203) [NBP2-52877] - Lane 1: Marker [kDa], Lane 2: RT-4

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in A431 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in HeLa cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in MCF7 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunocytochemistry/Immunofluorescence: Emerin Antibody (CL0203) [NBP2-52877] - Staining in U251 cell line with Anti-EMD monoclonal antibody, shows a distinct nuclear membranous pattern with a weaker additional ER staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human kidney shows nuclear membrane positivity in renal tubules.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human placenta shows strong nuclear membrane positivity in the trophoblast.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human prostate shows strong nuclear membrane immunoreactivityy in glandular cells.

Immunohistochemistry-Paraffin: Emerin Antibody (CL0203) [NBP2-52877] - Staining of human skin shows strong nuclear membrane positivity in the epidermis cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, Mycoplasma
• Clone: CL0203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Emerin Antibody (CL0203) - BSA Free Summary

• Description: Novus Biologicals Mouse Emerin Antibody (CL0203) - BSA Free (NBP2-52877) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Emerin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P50402, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EMD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Knockdown Validated
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Emerin Recombinant Protein Antigen (NBP2-52877PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for Emerin Antibody (CL0203) - BSA Free

• emerin
• Emery-Dreifuss muscular dystrophy
• LEM domain containing 5
• STAEDMDLEMD5

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Emerin Antibody (NBP2-52877)(1)

Publication using NBP2-52877

• Neggers JE, Paolella BR, Asfaw A et al. Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q Cell reports 2020-12-15 [PMID: 33326793] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: EMD