Human EMAP-II/AIMP1 ELISA Kit (Chemiluminescence)

ELISA: Human EMAP-II/AIMP1 ELISA Kit (Chemiluminescence) [NBP2-66678] - Samples were spiked with high concentrations of Human EMAP-II/AIMP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human EMAP-II/AIMP1 ELISA Kit (Chemiluminescence) [NBP2-66678] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Saliva, Urine, Milk, Tissue Homogenate
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human EMAP-II/AIMP1 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human AIMP1 in samples. No significant cross-reactivity or interference between Human AIMP1 and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 8.84%
• Intra-Assay: CV% < 10.96%
• Spike Recovery: 87-112%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: AIMP1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human EMAP-II/AIMP1 ELISA Kit (Chemiluminescence)

• AIMP1
• aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• EMAP-2
• EMAPII
• EMAP-II
• EMAPIImember 1 (endothelialmonocyte-activating)
• endothelial-monocyte activating polypeptide II
• Multisynthase complex auxiliary component p43
• multisynthetase complex auxiliary component p43
• SCYE1

Background

EMAP II (Endothelial-Monocyte-Activating Polypeptide II) is a proinflammatory cytokine and chemoattractant of macrophages and polymorphonuclear lymphocytes. EMAP II is synthesized as a 34 kDa precursor molecule which is cleaved to a biologically active 22 kDa mature polypeptide. This active protein is known to induce the procoagulant protein tissue factor on the surface of endothelial cells and modulate other properties of endothelial cells and monocytes in vitro, including the stimulation of TNF-alpha and myeloperoxidase. EMAP II activates neutrophils in vivo and induces an inflammatory reaction and tumor regression. EMAP II induces apoptosis in proliferating endothelial cells and inhibits neovascularization. This anti-angiogenic property is of interest to tumor biology. EMAP II is also a negative modulator of lung vascular growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for EMAP-II/AIMP1 ELISA Kit (NBP2-66678). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: AIMP1