Elongin A 293T Cell Transient Overexpression Lysate

Product Discontinued

Elongin A 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-TCEB3 antibody by Western Blots. Plasmid: pCMV-TCEB3 full-length
• Specificity: Elongin A 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: TCEB3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Elongin A 293T Cell Transient Overexpression Lysate

• EloA
• Elongin 110 kDa subunit
• elongin-A
• FLJ38760
• FLJ42849
• RNA polymerase II transcription factor SIII subunit A1
• SIII p110
• SIII
• TCEB3A
• transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A)
• transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
• transcription elongation factor B alpha subunit
• transcription elongation factor B polypeptide 3

Background

TCEB3 (Elongin A) is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TCEB3