Elongin A Antibody (1F3)

Western Blot: Elongin A Antibody (1F3) [H00006924-M02] - TCEB3 monoclonal antibody (M02), clone 1F3 Analysis of TCEB3 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: Elongin A Antibody (1F3) [H00006924-M02] - Analysis of monoclonal antibody to TCEB3 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: Elongin A Antibody (1F3) [H00006924-M02] - Analysis of TCEB3 expression in transfected 293T cell line by TCEB3 monoclonal antibody (M02), clone 1F3.Lane 1: TCEB3 transfected lysate(87.2 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Elongin A Antibody (1F3) Summary

• Immunogen: TCEB3 (NP_003189, 81 a.a. ~ 190 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NAEPDEQDFEKSNSRKRPRDALQKEEEMEGDYQETWKATGSRSYSPDHRQKKHRKLSELERPHKVSHGHERRDERKRCHRMSPTYSSDPESSDYGHVQSPPSCTSPHQMY
• Specificity: TCEB3 (1F3)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TCEB3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Elongin A Antibody (1F3)

• EloA
• Elongin 110 kDa subunit
• elongin-A
• FLJ38760
• FLJ42849
• RNA polymerase II transcription factor SIII subunit A1
• SIII p110
• SIII
• TCEB3A
• transcription elongation factor B (SIII), polypeptide 3 (110kD, elongin A)
• transcription elongation factor B (SIII), polypeptide 3 (110kDa, elongin A)
• transcription elongation factor B alpha subunit
• transcription elongation factor B polypeptide 3

Background

This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TCEB3
• Entrez:
  • 6924(Human)
• OMIM:
  • 600786(Human)
• Uniprot:
  • NP_003189(Human)