ELF3/ESE-1 Antibody - BSA Free

Western Blot: ESE1 Antibody [NB600-212] - WB detection of ESE1 in T84 colon carcinoma cells with NB600-212 antibody at a dilution of 1:500. Please note that this antibody detects both isoforms of ESE1 with expected molecular weight of 41.4 kDa and 39.3 kDa.

Western Blot: ESE1 Antibody [NB600-212] - Western blot analysis of ESE1 in T84 colon carcinoma cells using NB600-212 at 1:500 dilution. Recognizes both isoforms of ESE1.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ELF3/ESE-1 Antibody - BSA Free Summary

• Immunogen: N-terminal sequence and C-terminal sequence of the human ESE-1 protein
• Specificity: This recognizes both isoforms of ESE-1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ELF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: For Western Blotting, use at a dilution of 1:500 (ECL). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 21660457)

Reactivity Notes

Human. Others species have not been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ELF3/ESE-1 Antibody - BSA Free

• E74-like factor 3 (ets domain transcription factor, epithelial-specific )
• E74-like factor 3
• ELF3
• Epithelial-restricted with serine box
• epithelial-specific)
• Epithelium-restricted Ets protein ESX
• Epithelium-specific Ets transcription factor 1
• EPR1
• EPR-1
• ERT
• ESX
• ets domain transcription factor, serine box (epithelial-specific)
• ETS-related transcription factor Elf-3
• JEN
• serine box

Background

ESE-1 (Epithelium-Specific Ets) is a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, which has features distinct from those of any other ets-related factor. ESE-1 binds with high affinity to and transactivates the ets binding site in the promoter of the keratinocyte terminal differentiation marker gene, SPRR2A. ESE-1 is a critical regulator of epithelial cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ELF3/ESE-1 Antibody (NB600-212)(2)

Publications using NB600-212

• Marano RJ, Redmond SL. In vitro cultured primary cells from a human utricle explant possesses hair cell like characteristics. J Mol Histol 2011-08-01 [PMID: 21660457] (ICC/IF, Human)
• Ghassemifar R, Redmond S, Zainuddin, Chirila TV. Advancing towards a tissue-engineered tympanic membrane: silk fibroin as a substratum for growing human eardrum keratinocytes. J Biomater Appl;24(7):591-606. 2010-03-01 [PMID: 20308345] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: ELF3
• Entrez:
  • 1999(Human)