![Western Blot: Elastase Antibody (CELA3B/1811) - Azide and BSA Free [NBP2-54489] - Analysis of Recombinant Protein and Human Pancreas Lysates using CELA3B Monoclonal Antibody (CELA3B/1811)](http://images.novusbio.com/fullsize2/Elastase-Antibody-(CELA3B-1811)---Azide-and-BSA-Free-Western-Blot-NBP2-54489-img0001.jpg "Western Blot: Elastase Antibody (CELA3B/1811) - Azide and BSA Free [NBP2-54489] - Analysis of Recombinant Protein and Human Pancreas Lysates using CELA3B Monoclonal Antibody (CELA3B/1811)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, CyTOF-ready |
| Clone | CELA3B/1811 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant human CELA3B protein fragment (aa82-238) (exact sequence is proprietary) produced in E.coli |
| Localization | Cytoplasmic |
| Marker | Pancreatic Function Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CELA3B |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | ELISA (For coating order Ab without BSA) |
| Theoretical MW | 17 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-54489AF350 | |
| Alexa Fluor 405 | NBP2-54489AF405 | |
| Alexa Fluor 488 | NBP2-54489AF488 | |
| Alexa Fluor 532 | NBP2-54489AF532 | |
| Alexa Fluor 594 | NBP2-54489AF594 | |
| Alexa Fluor 647 | NBP2-54489AF647 | |
| Alexa Fluor 700 | NBP2-54489AF700 | |
| Alexa Fluor 750 | NBP2-54489AF750 | |
| Allophycocyanin | NBP2-54489APC | |
| Biotin | NBP2-54489B | |
| DyLight 350 | NBP2-54489UV | |
| DyLight 405 | NBP2-54489V | |
| DyLight 488 | NBP2-54489G | |
| DyLight 550 | NBP2-54489R | |
| DyLight 594 | NBP2-54489DL594 | |
| DyLight 650 | NBP2-54489C | |
| DyLight 680 | NBP2-54489FR | |
| DyLight 755 | NBP2-54489IR | |
| FITC | NBP2-54489F | |
| HRP | NBP2-54489H | |
| Janelia Fluor 549 | NBP2-54489JF549 | |
| Janelia Fluor 646 | NBP2-54489JF646 | |
| PE | NBP2-54489PE | |
| PerCP | NBP2-54489PCP |
Diseases for Elastase Antibody (NBP2-54489)Discover more about diseases related to Elastase Antibody (NBP2-54489).
| Pathways for Elastase Antibody (NBP2-54489)View related products by pathway.
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Research Areas for Elastase Antibody (NBP2-54489)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CELA3B |
![Flow Cytometry: Elastase Antibody (CELA3B/1811) - Azide and BSA Free [NBP2-54489] - Human bronchoalveolar lavage fluid. CF BALF cells stained for analysis by flow cytometry, gated on live neutrophils showing presence of NE on the cell surface. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize2/Elastase-Antibody-(CELA3B-1811)---Azide-and-BSA-Free-Flow-Cytometry-NBP2-54489-img0002.jpg "Flow Cytometry: Elastase Antibody (CELA3B/1811) - Azide and BSA Free [NBP2-54489] - Human bronchoalveolar lavage fluid. CF BALF cells stained for analysis by flow cytometry, gated on live neutrophils showing presence of NE on the cell surface. Flow cytometry image submitted by a verified customer review. Image from the Alexa Fluor 488 version of this antibody.")
![Western Blot: DBT Antibody [NBP1-89522] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/images2/DBT-Antibody-Western-Blot-NBP1-89522-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: DBT Antibody [NBP1-89522] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/images2/DBT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89522-img0007.jpg)
![Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon.](http://images.novusbio.com/images2/DBT-Antibody-Immunohistochemistry-Paraffin-NBP1-89522-img0015.jpg)
![Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/images2/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Western-Blot-NBP1-31302-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLD protein stained by DLD antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31302-img0013.jpg)
![Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Human gastric cancer, using DLD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/Dihydrolipoamide-Dehydrogenase-DLD-Antibody-Immunohistochemistry-Paraffin-NBP1-31302-img0008.jpg)
![Western Blot: ALDH9A1 Antibody [NBP1-89150] - Analysis in rat cell line NBT-II.](http://images.novusbio.com/images2/ALDH9A1-Antibody-Western-Blot-NBP1-89150-img0009.jpg)
![Immunohistochemistry-Paraffin: ALDH9A1 Antibody [NBP1-89150] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/images2/ALDH9A1-Antibody-Immunohistochemistry-Paraffin-NBP1-89150-img0002.jpg)
![Western Blot: ALDH9A1 Antibody [NBP1-89150] - Analysis in human cell line TD47D.](http://images.novusbio.com/images2/ALDH9A1-Antibody-Western-Blot-NBP1-89150-img0008.jpg)
![Western Blot: ILT6/CD85e/LILRA3 Antibody (2E9) [H00011026-M01] - Analysis of LILRA3 expression in transfected 293T cell line by LILRA3 monoclonal antibody (M01), clone 2E9.Lane 1: LILRA3 transfected lysate(50.485 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/ILT6-CD85e-LILRA3 Antibody (2E9)-Western Blot-H00011026-M01-img0002.jpg)
![Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Western-Blot-NBP1-58268-img0007.jpg)
![Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-NBP1-58268-img0008.jpg)
![Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue](http://images.novusbio.com/images2/Coagulation-Factor-II-Thrombin-Antibody-Immunohistochemistry-Paraffin-NBP1-58268-img0005.jpg)
![Western Blot: TTF2 Antibody (1E8) [H00008458-M06] - Analysis of TTF2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/images2/TTF2-Antibody-(1E8)-Western-Blot-H00008458-M06-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: TTF2 Antibody (1E8) [H00008458-M06] - Analysis of monoclonal antibody to TTF2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/TTF2-Antibody-(1E8)-Immunocytochemistry-Immunofluorescence-H00008458-M06-img0007.jpg)
![Western Blot: TTF2 Antibody (1E8) [H00008458-M06] - Analysis of TTF2 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/images2/TTF2-Antibody-(1E8)-Western-Blot-H00008458-M06-img0008.jpg)
![Western Blot: UBE4B Antibody [NBP1-32737] - (30 ug of whole cell lysate) A: Hep G2 5% SDS PAGE diluted at 1:10000](http://images.novusbio.com/images2/UBE4B Antibody-Western Blot-NBP1-32737-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: UBE4B Antibody [NBP1-32737] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/UBE4B Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32737-img0003.jpg)
![Immunohistochemistry-Paraffin: UBE4B Antibody [NBP1-32737] - Paraffin-embedded CA922 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/UBE4B Antibody-Immunohistochemistry-Paraffin-NBP1-32737-img0004.jpg)
![Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in NIH/3T3.](http://images.novusbio.com/images2/UBE4A-Antibody-(1G8)-Western-Blot-H00009354-M08-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of monoclonal antibody to UBE4A on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/UBE4A-Antibody-(1G8)-Immunocytochemistry-Immunofluorescence-H00009354-M08-img0002.jpg)
![Western Blot: UBE4A Antibody (1G8) [H00009354-M08] - Analysis of UBE4A expression in PC-12.](http://images.novusbio.com/images2/UBE4A-Antibody-(1G8)-Western-Blot-H00009354-M08-img0003.jpg)
![Immunohistochemistry-Paraffin: BBS9 Antibody [NBP1-88927] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/images2/BBS9-Antibody-Immunohistochemistry-Paraffin-NBP1-88927-img0002.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of alpha sarcoglycan on rat heart tissue lysates using anti-alpha sarcoglycan antibody at 1:500 dilution.](http://images.novusbio.com/images2/alpha-Sarcoglycan Antibody (JA51-81)-Western Blot-NBP2-67150-img0003.jpg)
![Immunohistochemistry-Frozen: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Human skeletal muscle tissue. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Frozen-NBP2-67150-img0005.jpg)
![Immunohistochemistry-Paraffin: alpha-Sarcoglycan Antibody (JA51-81) [NBP2-67150] - Analysis of paraffin-embedded mouse heart tissue using anti-alpha sarcoglycan antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/alpha-Sarcoglycan-Antibody-(JA51-81)-Immunohistochemistry-Paraffin-NBP2-67150-img0001.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - HeLa cells, concentration 3.3 ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0002.jpg)
![Immunohistochemistry: ATP6V0A2 Antibody [NBP1-59069] - Human kidney lysate tissue at an antibody concentration of 5.0 ug/ml using anti-ATP6V0A2 antibody](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Immunohistochemistry-NBP1-59069-img0008.jpg)
![Western Blot: ATP6V0A2 Antibody [NBP1-59069] - Hela tissue lysate at a concentration of 1ug/ml.](http://images.novusbio.com/images2/ATP6V0A2-Antibody-Western-Blot-NBP1-59069-img0001.jpg)
![Western Blot: GPHA2 Antibody [NBP3-04883] - Analysis of extracts of rat brain, using GPHA2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/images2/GPHA2 Antibody-Western Blot-NBP3-04883-img0001.jpg)
![SDS-Page: Human HR6B/UBE2B His (N-Term) Protein [NBP2-35088]](http://images.novusbio.com/images2/Human-HR6B-UBE2B-His-(N-Term)-Protein-SDS-Page-NBP2-35088-img0001.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-87 MG.](http://images.novusbio.com/images2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0007.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - SC65 directly interacts with lysyl-hydroxylase 1 (LH1). Western blot of primary calvarial osteoblast and skin fibroblast lysates from WT and Sc65KO 3 day-old mice (N = 2) showing significantly decreased levels of LH1 protein in Sc65KO samples. Densitometric quantification of LH1 protein normalized to beta-actin from the western blot shown above (*p<0.05; error bars represent SD). All experiments were performed at least 3 times. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1006002), licensed under a CC-BY licence.](http://images.novusbio.com/images2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0005.jpg)
![Western Blot: PLOD1 Antibody [NBP2-38770] - Analysis in human cell line U-251 MG and human cell line RT-4.](http://images.novusbio.com/images2/PLOD1-Antibody-Western-Blot-NBP2-38770-img0006.jpg)
![Western Blot: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of hnRNP C1+C2 on different lysates using anti-hnRNP C1+C2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: HepG2](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Western-Blot-NBP2-67656-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Staining hnRNP C1+C2 in B16-F1 (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/images2/hnRNP C1 + C2 Antibody (SN0652)-Immunocytochemistry-Immunofluorescence-NBP2-67656-img0010.jpg)
![Immunohistochemistry-Paraffin: hnRNP C1 + C2 Antibody (SN0652) [NBP2-67656] - Analysis of paraffin-embedded mouse skin tissue using anti-hnRNP C1+C2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/images2/hnRNP-C1-+-C2-Antibody-(SN0652)-Immunohistochemistry-Paraffin-NBP2-67656-img0008.jpg)
![Western Blot: BRD2 Antibody [NBP1-30475] - Detection of Human and Mouse BRD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody NBP1-30475 used for WB at 0.04 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/images2/BRD2-Antibody-Western-Blot-NBP1-30475-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: BRD2 Antibody [NBP1-30475] - Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 used at a dilution of 1:100 (2 ug/mL) (left) and rabbit anti-BRD2 recombinant monoclonal used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.](http://images.novusbio.com/images2/BRD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30475-img0021.jpg)
![Immunohistochemistry-Paraffin: BRD2 Antibody [NBP1-30475] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (NBP1-30475). Detection: DAB](http://images.novusbio.com/images2/BRD2-Antibody-Immunohistochemistry-Paraffin-NBP1-30475-img0020.jpg)
