Elastase Antibody (CELA3B/1218)

Images

 
Western Blot: Elastase Antibody (CELA3B/1218) [NBP2-44812] - Western Blot Analysis of human PANC-1 cell lysate using Elastase Antibody (CELA3B/1218)
Western Blot: Elastase Antibody (CELA3B/1218) [NBP2-44812] - Western Blot of Recombinant, Panc-28 and PANC1 cell lysates using Elastase Antibody (CELA3B/1218).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
CELA3B/1218
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Elastase Antibody (CELA3B/1218) Summary

Immunogen
Recombinant human Elastase protein fragment (around aa 82-238) (exact sequence is proprietary) (Uniprot: P08861)
Localization
Cytoplasmic
Marker
Pancreatic Function Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CELA3B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 2-4 ug/ml
  • Western Blot 2-4 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Optimal dilution for a specific application should be determined.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47938)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Elastase Antibody (CELA3B/1218)

  • CBPP
  • cholesterol-binding pancreatic protease
  • chymotrypsin-like elastase family member 3B
  • chymotrypsin-like elastase family, member 3B
  • E1
  • EC 3.4.21
  • EC 3.4.21.70
  • EL-1
  • ELA3B
  • elastase 1
  • elastase 3B, pancreatic
  • Elastase IIIB
  • elastase-3B
  • fecal elastase 1
  • pancreatic elastase 1
  • pancreatic endopeptidase E
  • Protease E
  • proteinase E

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike otherelastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted fromthe pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has adigestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3Bmay also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B havebeen referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently usedas a measure of pancreatic function in clinical assays. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CELA3B
Uniprot