ELA3A Antibody

Western Blot: ELA3A Antibody [NBP2-14946] - Sample (30 ug of whole cell lysate) A: PC-3 12% SDS PAGE, antibody diluted at 1:2000.

Immunohistochemistry-Paraffin: ELA3A Antibody [NBP2-14946] - Paraffin-embedded human esophagus carcinoma. ELA3A antibody diluted at 1:500.

Immunohistochemistry-Paraffin: ELA3A Antibody [NBP2-14946] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ELA3A Antibody Summary

• Description: Novus Biologicals Rabbit ELA3A Antibody (NBP2-14946) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ELA3A. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CELA3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ELA3A Antibody

• chymotrypsin-like elastase family member 3A
• chymotrypsin-like elastase family, member 3A
• EC 3.4.21
• EC 3.4.21.70
• ELA3A
• ELA3elastase 1
• elastase 3A, pancreatic (protease E)
• elastase 3A, pancreatic
• Elastase IIIA
• elastase-3A
• Protease E

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CELA3A
• Uniprot:
  • P09093(Human)