CELA1 Antibody (4H5) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: CELA1 Antibody (4H5) [H00001990-M02] - Analysis of monoclonal antibody to ELA1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CELA1 Antibody (4H5) [H00001990-M02] - Analysis of monoclonal antibody to ELA1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml.

Sandwich ELISA: CELA1 Antibody (4H5) [H00001990-M02] - Detection limit for recombinant GST tagged ELA1 is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4H5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CELA1 Antibody (4H5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ELA1 (NP_001962.3, 159 a.a. ~ 257 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LAQTLQQAYLPSVDYAICSSSSYWGSTVKNTMVCAGGDGVRSGCQGDSGGPLHCLVNGKYSVHGVTSFVSSRGCNVSRKPTVFTQVSAYISWINNVIAS
• Specificity: Reacts with elastase 1, pancreatic.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CELA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in WB and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CELA1 Antibody (4H5) - Azide and BSA Free

• chymotrypsin-like elastase family member 1
• chymotrypsin-like elastase family, member 1
• EC 3.4.21
• EC 3.4.21.36
• ELA1elastase-1
• elastase 1, pancreatic
• Elastase-1

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, pancreatic elastase 1 is not expressed in the pancreas. To date, elastase 1 expression has only been detected in skin keratinocytes. Clinical literature that describes human elastase 1 activity in the pancreas or fecal material is actually referring to chymotrypsin-like elastase family, member 3B. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CELA1 Antibody (H00001990-M02) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/07/2023

Summary

• Application: Western Blot
• Sample Tested: Human pancreas/duct epithelioid carcinoma cell line - PANC1 Cells (ATCC # CRL-1469)
• Species: Human
• Lot: D4291-4H5

Bioinformatics

• Gene Symbol: CELA1
• Entrez:
  • 1990(Human)
• Uniprot:
  • NP_001962(Human)