eIF4B Antibody (866049) [Alexa Fluor® 405]

• Applications: WB
• Clone: 866049
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

eIF4B Antibody (866049) [Alexa Fluor® 405] Summary

• Immunogen: Phosphopeptide containing the human eIF4B S422 site
• Specificity: Detects human eIF4B when phosphorylated at S422
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

eIF4B (eukaryotic translation initiation factor 4B), an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with the heterotrimeric eIF4F, stimulates the ATPase and RNA helicase activity of eIF4E.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.