EIF3S3 Overexpression Lysate (Native)

Images

 
Western Blot: EIF3S3 Overexpression Lysate (Adult Normal) [NBL1-10196] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EIF3S3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EIF3S3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201767 and based on accession number NM_003756. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens eukaryotic translation initiation factor 3, subunit H (EIF3H), mRNA.
Gene
EIF3H

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
39.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EIF3S3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EIF3S3 Overexpression Lysate (Native)

  • eIF-3-gamma
  • eIF3-gamma
  • eIF3h
  • eIF3-p40
  • EIF3S3eIF3 p40 subunit
  • Eukaryotic translation initiation factor 3 subunit 3
  • eukaryotic translation initiation factor 3 subunit H
  • eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD)
  • eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD)
  • eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa
  • eukaryotic translation initiation factor 3, subunit H
  • MGC102958

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP2-38612
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84022
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
409-ML/CF
Species: Mu
NBP1-77997
Species: Hu, Mu, Rt, Bv, Ch, Pm
Applications: WB, ELISA, IHC, IHC-P
NBP1-81796
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-21577
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-33002
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-32956
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-33090
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NBP1-84869
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91875
Species: Hu
Applications: WB, IHC, IHC-P
NB100-56603
Species: Hu, Pm
Applications: WB, IHC, IHC-P
NBP1-18891
Species: Hu, Mu
Applications: WB, IP
AF4838
Species: Hu
Applications: WB, Simple Western
AF748
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-16468
Species: Hu, Rt
Applications: WB, IHC, IHC-P
NBP1-98293
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP

Publications for EIF3S3 Lysate (NBL1-10196) (0)

There are no publications for EIF3S3 Lysate (NBL1-10196).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EIF3S3 Lysate (NBL1-10196) (0)

There are no reviews for EIF3S3 Lysate (NBL1-10196). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EIF3S3 Lysate (NBL1-10196) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional EIF3S3 Products

Bioinformatics Tool for EIF3S3 Lysate (NBL1-10196)

Discover related pathways, diseases and genes to EIF3S3 Lysate (NBL1-10196). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EIF3S3 Lysate (NBL1-10196)

Discover more about diseases related to EIF3S3 Lysate (NBL1-10196).
 

Pathways for EIF3S3 Lysate (NBL1-10196)

View related products by pathway.

PTMs for EIF3S3 Lysate (NBL1-10196)

Learn more about PTMs related to EIF3S3 Lysate (NBL1-10196).
 

Research Areas for EIF3S3 Lysate (NBL1-10196)

Find related products by research area.

Blogs on EIF3S3

There are no specific blogs for EIF3S3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

c-Myc Antibody
NB600-335
EIF3S3 Antibody
NBP1-84870

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EIF3S3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol EIF3H