EIF3D Overexpression Lysate

Images

 
Western Blot: EIF3D Overexpression Lysate (Adult Normal) [NBL1-10191] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EIF3D.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EIF3D Overexpression Lysate Summary

Description
Transient overexpression lysate of eukaryotic translation initiation factor 3, subunit D (EIF3D) The lysate was created in HEK293T cells, using Plasmid ID RC203830 and based on accession number NM_003753. The protein contains a C-MYC/DDK Tag.
Gene
EIF3D

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
63.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EIF3D Overexpression Lysate

  • eIF3d
  • eIF3-p66
  • EIF3S7eIF3 p66
  • eIF-3-zeta
  • eIF3-zeta
  • Eukaryotic translation initiation factor 3 subunit 7
  • eukaryotic translation initiation factor 3 subunit D
  • eukaryotic translation initiation factor 3, subunit 7 zeta, 66/67kDa
  • eukaryotic translation initiation factor 3, subunit D
  • MGC126526
  • MGC17258
  • translation initiation factor eIF3 p66 subunit

Background

Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-84869
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-16299
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, ChIP
NBP1-84870
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-18891
Species: Hu, Mu
Applications: WB, IP
NBP2-24571
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
H00010714-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-85302
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-84873
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, KD
NBP2-24524
Species: Hu, Mu, Rt, Bv, Ca, Eq, Pm
Applications: WB, IHC, IHC-P
H00058503-P01
Species: Hu
24790002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-06556
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IP
NB100-511
Species: Hu, Mu
Applications: WB, IP
NB500-317
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
NBP2-19676
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-495
Species: Hu, Mu, Rt, Dr
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single Cell Western
NBP1-60103
Species: Hu
Applications: WB, IHC
NBP2-13954
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-10191
Species: Hu
Applications: WB

Publications for EIF3D Lysate (NBL1-10191) (0)

There are no publications for EIF3D Lysate (NBL1-10191).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EIF3D Lysate (NBL1-10191) (0)

There are no reviews for EIF3D Lysate (NBL1-10191). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EIF3D Lysate (NBL1-10191) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-10191

Additional EIF3D Products

Bioinformatics Tool for EIF3D Lysate (NBL1-10191)

Discover related pathways, diseases and genes to EIF3D Lysate (NBL1-10191). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EIF3D Lysate (NBL1-10191)

Discover more about diseases related to EIF3D Lysate (NBL1-10191).
 

Pathways for EIF3D Lysate (NBL1-10191)

View related products by pathway.

PTMs for EIF3D Lysate (NBL1-10191)

Learn more about PTMs related to EIF3D Lysate (NBL1-10191).

Blogs on EIF3D

There are no specific blogs for EIF3D, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EIF3D Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol EIF3D
COVID-19 update