EIF2B2 Antibody

Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-EIF2B2 )

Product Discontinued

EIF2B2 Antibody Summary

• Immunogen: The immunogen for this antibody was a sequence from 1a.a. to 210a.a. from EIF2B2(a 351a.a. long protein)
• Specificity: EIF2B2
• Clonality: Polyclonal
• Host: Chicken
• Gene: EIF2B2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: ELISA: Direct Elisa Test. Protein as test antigen. Affi-pure IgY as primary antibody and Goat anti-IgY HRP as 2nd antibody. Fixed amount of antibody (1 ug/ml) and serial dilutions of antigen.

Reactivity Notes

This antibody is reactive against Human, mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for EIF2B2 Antibody

• eIF-2B GDP-GTP exchange factor subunit beta
• EIF2B
• EIF2BB
• EIF-2Bbeta
• eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD)
• eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa
• S20I15
• S20III15
• translation initiation factor eIF-2B subunit beta

Background

Eukaryotic initiation factor 2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha, beta, gamma, delta and epsilon subunits. EIF2B activates its EIF2 substrate by exchanging EIF2 bound GDP for GTP. Defects in EIF2B2 are a cause of leukoencephalopathy with vanishing white matter (VWM). VWM is a brain disease that occurs mainly in children. The neurological signs of VWM include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. Head trauma leads only to motor deterioration whereas infections with fever may end in coma. Death occurs after a variable period of a few years to a few decades, usually following an episode of fever and coma. Defects in EIF2B2 are a cause of ovarioleukodystrophy; also known as vanishing white matter leukodystrophy with ovarian failure. It is a condition of ovarian failure associated with cerebral abnormalities similar to those in patients with vanishing white matter leukodystrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2B2
• Entrez:
  • 8892(Human)
  • 217715(Mouse)
• Uniprot:
  • P49770(Human)