![Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunohistochemistry-NBP1-30449-img0003.jpg "Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
| Reactivity | Hu, RbSpecies Glossary |
| Applications | IHC, IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit eIF2B epsilon Antibody - BSA Free (NBP1-30449) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen for this product maps to a region between residue 550 and 600 of human eukaryotic translation initiation factor 2B, subunit 5 epsilon using the numbering given in entry NP_003898.2 (GeneID 8893). |
| Predicted Species | Rabbit (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EIF2B5 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for eIF2B epsilon Antibody (NBP1-30449)Find related products by research area.
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| Gene Symbol | EIF2B5 |
![Immunoprecipitation: eIF2B epsilon Antibody [NBP1-30449] - Detection of Human eIF2B5 Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody NBP1-30449 used for IP at 10 mcg/mg lysate. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30448, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunoprecipitation-NBP1-30449-img0001.jpg "Immunoprecipitation: eIF2B epsilon Antibody [NBP1-30449] - Detection of Human eIF2B5 Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody NBP1-30449 used for IP at 10 mcg/mg lysate. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30448, which recognizes an upstream epitope.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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