| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35116-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21973-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56363-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56270-25ul | |||
![Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-5M2D4-Western-Blot-NBP3-16674-img0006.jpg "Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16674_rabbit-eif2b-epsilon-mab-5m2d4-immunohistochemistry-paraffin-107202482855..jpg)
![Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16674_rabbit-eif2b-epsilon-mab-5m2d4-immunohistochemistry-paraffin-10720248301..jpg)
![Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human colon tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16674_rabbit-eif2b-epsilon-mab-5m2d4-immunohistochemistry-paraffin-10720248319..jpg)
![Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human breast cancer tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16674_rabbit-eif2b-epsilon-mab-5m2d4-immunohistochemistry-paraffin-107202483227..jpg)
![Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Confocal imaging of MCF7 cells using eIF2B epsilon Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16674_rabbit-eif2b-epsilon-mab-5m2d4-822025208429.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP1-32783] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Western-Blot-NBP1-32783-img0005.jpg "Western Blot: eIF2B epsilon Antibody [NBP1-32783] - Sample (30 ug of whole cell lysate)A: H12997. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody [NBP1-32783] - Paraformaldehyde-fixed A431, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32783-img0003.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP1-32783] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Western-Blot-NBP1-32783-img0004.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP1-30448] - Western Blot and Immunoprecipitation: Detection of Human eIF2B5 Whole cell lysate from HeLa and 293 cells. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30449.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Western-Blot-NBP1-30448-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: eIF2B epsilon Antibody [NBP1-30448] - Western Blot and Immunoprecipitation: Detection of Human eIF2B5 Whole cell lysate from HeLa and 293 cells. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30449.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunohistochemistry-NBP1-30448-img0005.jpg)
![Immunoprecipitation: eIF2B epsilon Antibody [NBP3-35116] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells using 3 ug eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263). Western blot was performed from the immunoprecipitate using eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263) at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-35116_rabbit-eif2b-epsilon-pab-912202414154314.jpg "Immunoprecipitation: eIF2B epsilon Antibody [NBP3-35116] - Immunoprecipitation analysis of 200 ug extracts of A-549 cells using 3 ug eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263). Western blot was performed from the immunoprecipitate using eIF2B epsilon(eIF2B epsilon(EIF2B5)) antibody ( A10263) at a dilution of 1:1000.")
![Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody [NBP3-35116] - Immunofluorescence analysis of L929 cells using eIF2B epsilon(eIF2B epsilon(EIF2B5)) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35116_rabbit-eif2b-epsilon-pab-912202415161839.jpg)
![Western Blot: eIF2B epsilon Antibody [NBP3-35116] - Western blot analysis of various lysates using eIF2B epsilon(EIF2B5) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35116_rabbit-eif2b-epsilon-pab-91220241519514.jpg)
![Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunohistochemistry-NBP1-30449-img0003.jpg "Immunohistochemistry-Paraffin: eIF2B epsilon Antibody [NBP1-30449] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunoprecipitation: eIF2B epsilon Antibody [NBP1-30449] - Detection of Human eIF2B5 Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-eIF2B5 antibody NBP1-30449 used for IP at 10 mcg/mg lysate. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30448, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunoprecipitation-NBP1-30449-img0001.jpg)
![Western Blot: eIF2B epsilon Antibody (SR2229) [NBP3-21973] - Western blot analysis using NBP3-21973 in Hela cell lysate.](http://images.novusbio.com/fullsize/nbp3-21973_rabbit-eif2b-epsilon-mab-sr2229-57202316112135.jpg "Western Blot: eIF2B epsilon Antibody (SR2229) [NBP3-21973] - Western blot analysis using NBP3-21973 in Hela cell lysate.")
![Immunohistochemistry:[NBP3-29291]-Detection of human eIF2B5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5.](http://images.novusbio.com/fullsize/nbp3-29291_rabbit-eif2b-epsilon-pab-2312025128819.jpg "Immunohistochemistry:[NBP3-29291]-Detection of human eIF2B5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5.")
![Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody [NBP2-56363] - Staining of human cell line RT4 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56363-img0001.jpg "Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody [NBP2-56363] - Staining of human cell line RT4 shows localization to cytosol. Antibody staining is shown in green.")
![Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody [NBP2-56270] - Staining of human cell line MCF7 shows localization to cytosol.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56270-img0001.jpg "Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody [NBP2-56270] - Staining of human cell line MCF7 shows localization to cytosol.")
![Western Blot: eIF2B epsilon Overexpression Lysate (Adult Normal) [NBL1-10181] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF2B epsilon.](http://images.novusbio.com/fullsize/eIF2B-epsilon-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10181-img0002.jpg "Western Blot: eIF2B epsilon Overexpression Lysate (Adult Normal) [NBL1-10181] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF2B epsilon.")