eIF2A [p Ser51] Antibody

Images

 
Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.
Immunocytochemistry/ Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed Hela cells using eIF2a(Phospho-Ser51) antibody.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.
Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using eIF2 alpha (phospho-Ser51) antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

eIF2A [p Ser51] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human eIF2. around the phosphorylation site of serine 51 (E-L-SP-R-R).
Modification
p Ser51
Specificity
eIF2. (specific to Phospho-Ser51) detects endogenous levels of eIF2. only when phosphorylated at serine 51.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
eIF2A Lysate (NBL1-10174)
Publications
Read Publication using
NB100-81896 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF2A [p Ser51] Antibody

  • EIF-2
  • EIF-2A
  • EIF2AEIF2
  • EIF-2alpha
  • eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • subunit 1 (alpha, 35kD )

Background

eIF2 alpha is a 36 kDa protein which is ubiquitously expressed in many cell types. The eIF2 protein, which is composed of three subunits (alpha, beta and gamma), is one of the key molecules in the initiation of translation. In mammalian cells, eIF2 alpha is phosphorylated at serine 51 (human EIF2 alpha, the equivalent residue in mouse is serine 52) by at least two kinases: the haem-controlled repressor (HCR) and the interferon inducible double stranded RNA-dependent protein kinase (PKR). Phosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important regulatory process in protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3999
Species: Hu
Applications: WB, KO
NBP1-40256
Species: Hu, Mu, Rt, Po, Fi, Ha, Rb
Applications: WB, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB600-1335
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, GS, ICC/IF, IHC, IHC-P, IP
NBP1-06274
Species: Hu, Mu, Rt, Ch, Dr, Sh
Applications: WB, Simple Western, Flow, ICC/IF
NBP1-77266
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
AF8519
Species: Hu, Mu, Rt
Applications: WB
NBP2-02669
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF835
Species: Hu, Mu
Applications: ICC
NBP1-77681
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-2323
Species: Hu, Mu, Rt, Po, Ma, Mk, Pm, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01787
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB110-41536
Species: Hu, Mu
Applications: WB, ICC/IF
NBP2-37620
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC
NBP1-83139
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-86960
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF3795
Species: Hu
Applications: WB, IP
NBP2-37474
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready

Publications for eIF2A Antibody (NB100-81896)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for eIF2A Antibody (NB100-81896) (0)

There are no reviews for eIF2A Antibody (NB100-81896). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for eIF2A Antibody (NB100-81896) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for eIF2A Antibody (NB100-81896)

Discover related pathways, diseases and genes to eIF2A Antibody (NB100-81896). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for eIF2A Antibody (NB100-81896)

Discover more about diseases related to eIF2A Antibody (NB100-81896).
 

Pathways for eIF2A Antibody (NB100-81896)

View related products by pathway.

PTMs for eIF2A Antibody (NB100-81896)

Learn more about PTMs related to eIF2A Antibody (NB100-81896).
 

Research Areas for eIF2A Antibody (NB100-81896)

Find related products by research area.

Blogs on eIF2A.

eIF2alpha - a regulator of global translation in response to cellular stress
Eukaryotic initiation factor 2 (eIF2) regulates global protein translation by binding to Met-tRNA and the 40S ribosome to form the pre-initiation complex. eIF2 is a heterotrimer consisting of alpha, beta, and gamma subunits. The 36kDA eIF2a subuni...  Read full blog post.

PKR - Mediating cellular stress responses through multiple signaling pathways
Protein kinase R (PKR) is an intracellular stress-sensing protein that is able to detect and respond to viral infections. While PKR is able to sense and respond to a variety of signals, dsRNA is a well-characterized ligand. dsRNA produced during v...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our eIF2A [p Ser51] Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol EIF2A
Entrez
Uniprot