eIF2A Antibody (3A7B11) - BSA Free

Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.

Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using eIF2A (3A7B11) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and perinuclear staining was observed. Staining was performed by Histowiz.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: 3A7B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

eIF2A Antibody (3A7B11) - BSA Free Summary

• Description: Novus Biologicals Mouse eIF2A Antibody (3A7B11) - BSA Free (NBP2-26296) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Partial recombinant human EIF2A (between residues 350-585) expressed in E. coli [Uniprot Q9BY44]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF2A
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% proprietary protein stabilizer
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for eIF2A Antibody (3A7B11) - BSA Free

• EIF-2
• EIF-2A
• EIF2AEIF2
• EIF-2alpha
• eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
• subunit 1 (alpha, 35kD )

Background

eIF-2 alpha (also known as eukaryotic translation initiation factor 2, subunit 1 alpha) is a 35 kD member of the eukaryotic translation initiation factor 2 family. This cytoplasmic protein is ubiquitously expressed and can downregulate protein synthesis when phosphorylated by PKR kinase under stress conditions (hypoxia, viral infection, ER stress). Increased expression of eIF-2 alpha has been linked to progression of thyroid carcinoma.Signaling inhibited by p58 (IPK) and PKR phosphorylation. Can attenuate the unfolded protein response and inhibits translation. This protein has been shown to interact with eIF2B alpha, eIF2B delta, eIF2B beta subunit 2, p67, p68, and PKR. eIF-2 alpha is a target for cleavage by caspase 3, caspase 6, caspase 8, and caspase 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF2A
• Entrez:
  • 83939(Human)
• Uniprot:
  • Q9BY44(Mouse)