EG-VEGF/PK1 Antibody (239727) [Unconjugated]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 239727
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

EG-VEGF/PK1 Antibody (239727) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat EG-VEGF
  Ala20-Phe105 (predicted)
  Accession # Q8R414
• Specificity: Detects human, mouse, and rat EG-VEGF in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human EG-VEGF and recombinant mouse EG-VEGF is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PROK1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EG-VEGF/PK1 Antibody (239727) [Unconjugated]

• EGVEGF
• EG-VEGF
• EGVEGFblack mamba toxin-related protein
• Endocrine-gland-derived vascular endothelial growth factor
• Mambakine
• PK1mambakine
• PRK1prokineticin-1
• PROK1
• Prokineticin 1

Background

EG-VEGF, a member of the prokineticin family of secreted proteins, is expressed in multiple tissues including the gastrointestinal tract, testis, ovary, placenta and adrenal glands. It binds to the G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2, leading to stimulation of smooth muscle contraction and tissue‑specific angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EG-VEGF/PK1 Antibody (MAB2100)(1)

Publication using MAB2100

• Urayama K, Guilini C, Messaddeq N, Hu K, Steenman M, Kurose H, Ert G, Nebigil CG The prokineticin receptor-1 (GPR73) promotes cardiomyocyte survival and angiogenesis. FASEB J., 2007-04-18;21(11):2980-93. 2007-04-18 [PMID: 17442730] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: PROK1
• Uniprot:
  • Q8R414()