EEF1B2 Antibody - BSA Free

Western Blot: EEF1B2 Antibody [NBP2-92964] - Analysis of extracts of various cell lines, using EEF1B2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-92964] - Paraffin-embedded rat spinal cord using EEF1B2 .

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-92964] - Paraffin-embedded mouse brain using EEF1B2 .

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-92964] - Paraffin-embedded rat brain using EEF1B2 .

Western Blot: EEF1B2 Antibody [NBP2-92964] - Analysis of extracts from normal (control) and EEF1B2 knockout (KO) 293T cells, using EEF1B2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in T

Immunocytochemistry/ Immunofluorescence: EEF1B2 Antibody - BSA Free [NBP2-92964] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] EEF1B2 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EEF1B2 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit EEF1B2 Antibody - BSA Free (NBP2-92964) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1). MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EEF1B2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot 1:500-1:2000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EEF1B2 Antibody - BSA Free

• EEF1B
• EEF1B1
• EF1B
• EF-1-beta
• elongation factor 1-beta
• eukaryotic translation elongation factor 1 beta 1
• eukaryotic translation elongation factor 1 beta 2

Background

EEF1B2 encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EEF1B2