EDF1 Antibody (3E6) - Azide and BSA Free

Western Blot: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of EDF1 expression in Hela S3 NE (Cat # L013V3).

Immunocytochemistry/Immunofluorescence: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of monoclonal antibody to EDF1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: EDF1 Antibody (3E6) [H00008721-M03] - Analysis of monoclonal antibody to EDF1 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml

Sandwich ELISA: EDF1 Antibody (3E6) [H00008721-M03] - Detection limit for recombinant GST tagged EDF1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3E6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EDF1 Antibody (3E6) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EDF1 (AAH15500.1, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAESDWDTVTVLRKKGPTAAQAKSKQAILAAQSRGEDVETSKKWAAGQNKQHSITKNTAKLDRETEELHHDRVTLEVGKVIQQGRQSKGLTQKDLATKINEKPQVIADYESGRAIPNNQVLGKIERAIGLKLRGKDIGKPIEKGPRAK
• Specificity: Reacts with endothelial differentiation-related factor 1.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EDF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate in WB and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EDF1 Antibody (3E6) - Azide and BSA Free

• EDF-1Multiprotein-bridging factor 1
• endothelial differentiation-related factor 1
• MBF1
• MGC9058
• multiprotein bridging factor 1
• multiprotein bridging factor-1

Background

This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two alternatively spliced transcripts which encode distinct proteins have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDF1
• Entrez:
  • 8721(Human)
• Uniprot:
  • AAH15500(Human)