EDEM2 Antibody

Western Blot: EDEM2 Antibody [NBP2-97100] - Anti-EDEM2 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa

Immunohistochemistry-Paraffin: EDEM2 Antibody [NBP2-97100] - Immunochemical staining of human EDEM2 in human small intestine with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: EDEM2 Antibody [NBP2-97100] - EDEM2 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-EDEM2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein G. Primary antibody: Anti-EDEM2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 65 kDa

Product Discontinued

EDEM2 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human EDEM2 (AAH01371.1; Met1-Lys492).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDEM2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Affinity purified

Alternate Names for EDEM2 Antibody

• bA4204.1
• C20orf31
• C20orf49
• chromosome 20 open reading frame 31
• ER degradation enhancer, mannosidase alpha-like 2
• ER degradation-enhancing alpha-mannosidase-like 2
• ER degradation-enhancing-mannosidase-like protein 2
• FLJ10783

Background

In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EDEM2