EDEM2 Antibody (2E4)

Images

 
Western Blot: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 expression in transfected 293T cell line by C20orf31 monoclonal antibody (M01), clone 2E4.Lane 1: EDEM2 transfected lysate(64.8 KDa).Lane 2: ...read more
Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of monoclonal antibody to C20orf31 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.
ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged C20orf31 is approximately 30ng/ml as a capture antibody.
Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 transfected lysate using anti-EDEM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDEM2 MaxPab rabbit polyclonal ...read more
Sandwich ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged EDEM2 is 0.1 ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IP
Clone
2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

EDEM2 Antibody (2E4) Summary

Immunogen
C20orf31 (NP_060687, 479 a.a. ~ 579 a.a) partial recombinant protein with GST tag.PIDPAALHCCQRLKEEQWEVEDLMREFYSLKRCRSKFQKNTVSSGPWEPPARPGTLFSPENHDQARERKPAKQKVPLLSCPSQPFTSKLALLGQVFLDSS
Specificity
C20orf31 - chromosome 20 open reading frame 31
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EDEM2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EDEM2 Antibody (2E4)

  • bA4204.1
  • C20orf31
  • C20orf49
  • chromosome 20 open reading frame 31
  • ER degradation enhancer, mannosidase alpha-like 2
  • ER degradation-enhancing alpha-mannosidase-like 2
  • ER degradation-enhancing-mannosidase-like protein 2
  • FLJ10783

Background

In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). EDEM2 belongs to a family of proteins involved in ERAD of glycoproteins (Mast et al., 2005 [PubMed 15537790]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol EDEM2
Entrez
Uniprot