EDA-A2/Ectodysplasin A2 Antibody [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor Plus 555

EDA-A2/Ectodysplasin A2 Antibody [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human EDA‑A2/Ectodysplasin A2 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhLIGHT, rhOX40 Ligand, and rhTRAIL is observed and less than 2% cross‑reactivity with recombinant mouse EDA, rhTNF-alpha , rhVEGI, rhGITR Ligand, and rhFasLigand is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EDA-A2/Ectodysplasin A2 Antibody [Alexa Fluor™ Plus 555]

• ECTD1;Ectodysplasin-A;ED1;ED1-A1;ED1-A2;EDA1;EDA2;EDA-A1;EDA-A2;HED;HED1;ODT1;STHAGX1;TNLG7C;XHED;XLHED
• EDAA2
• EDA-A2

Background

EDA-A2, a product of the EDA gene (also called Tabby), is a type II transmembrane protein that is a member of the TNF Superfamily (TNFSF). Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. The extracellular domains of human and mouse EDA-A2 share approximately 94% identity. Within the TNFSF, EDA-A2 shares the highest homology with EDA-A1, the other product of the EDA gene. EDA-A2 and EDA-A1 are splice variants of EDA which differ by only two amino acids. EDA-A1 contains two additional amino acids, Glu308 and Val309. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds EDAR, a member of the TNF Receptor Superfamily (TNFRSF), whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF family member with high homology to EDAR. Mutations in EDA-A1, EDA-A2, EDAR and XEDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene.

1. Bayes, M. et al. (1998) Hum. Mol. Genet. 7:1661.
2. Kere, J. et al. (1996) Nat. Genet. 13:409.
3. Monreal, A.W. et al. (1998) Am. J. Hum. Genet. 63:380.
4. Headon, D.J. and P.A. Overbeek (1999) Nat. Genet. 22(4):370.
5. Kumar, A. et al. (2000) J. Biol. Chem. 276(4):2668.
6. Monreal, A.W. et al. (1999) Nat. Genet. 22(4):366.
7. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94(24):13069.
8. Yan, M. et al. (2000) Science 290(5491):523.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.