Recombinant Human ECSIT His Protein

SDS-Page: ECSIT Protein [NBP2-23017]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human ECSIT His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 19-217 of Human ECSIT
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSGTCGAAL TGTSISQVPL PKDSTGAADP PQPHIVGIQS PDQQAALARH NPARPVFVEG PFSLWLRNKC VYYHILRADL LPPEEREVEE TPEEWNLYYP MQLDLEYVRS GWDNYEFDIN EVEEGPVFAM CMAGAHDQAT MAKWIQGLQE TNPTLAQIPV VFRLAGSTRE LQTSSAGLEE PPLPEDHQEE DDNLQRQQQG QS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ECSIT
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 24.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human ECSIT His Protein

• ECSIT homolog (Drosophila)
• evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial
• likely ortholog of mouse signaling intermediate in Toll pathway evolutionarilyconserved
• Protein SITPEC
• signaling intermediate in Toll pathway evolutionarily conserved ortholog
• SITPECsignaling intermediate in Toll pathway, evolutionarily conserved

Background

ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ECSIT