ECE-2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ECE-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ECE-2
  Gly104-Trp787
  Accession # EAW78275
• Specificity: Detects human ECE-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human ECE-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ECE2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ECE-2 Antibody [Unconjugated]

• EC 3.4.24
• EC 3.4.24.71
• ECE2
• ECE-2
• endothelin converting enzyme 2
• endothelin-converting enzyme 2
• KIAA0604MGC2408
• MGC17664
• MGC78487

Background

ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.

1. Turner, A.J. et al. (2001) BioEssays 23:261.
2. Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
3. Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
4. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ECE-2 Antibody (AF1645)(2)

Publications using AF1645

• JS Miners, S Love Endothelin-converting enzymes degrade ?-synuclein and are reduced in dementia with Lewy bodies J. Neurochem, 2017-03-29;0(0):. 2017-03-29 [PMID: 28171705] (ICC, Human)
• Palmer JC, Baig S, Kehoe PG, Love S Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta. Am. J. Pathol., 2009-06-18;175(1):262-70. 2009-06-18 [PMID: 19541930] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: ECE2
• Uniprot:
  • EAW78275()