EAAT2/GLT1 Antibody

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP2-68147] - Sections of mouse olfactory bulbs. Dilution 1:1000.

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP2-68147] - Sections of mouse brain (hippocampus). Dilution 1: 1000.

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP2-68147] - Sections of mouse spinal cord. Dilution 1:1000

Immunohistochemistry-Paraffin: EAAT2/GLT1 Antibody [NBP2-68147] - Sections of mouse spinal cord. Dilution 1:1000.

Product Discontinued

EAAT2/GLT1 Antibody Summary

• Immunogen: A synthetic peptide from extracellular domain of mouse Excitatory amino acid transporter 2 (EAAT2, EAA2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and human.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC1A2
• Purity: Whole Antiserum

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • In vivo assay
  • Western Blot 1:300 - 1:2000
• Application Notes: Although not tested, this antibody may work in Immunohistochemistry-Frozen. Use in FLOW was reported in the scientific literature (PMID: 23793269). Use in In-vivo and in iCC/IF reported in scientific literature (PMID 25581361).

Packaging, Storage & Formulations

• Storage: Store at -20C as supplied. After reconstitution, store at 2C to 8C for 1 month and at -20 for long term storage. Avoid repeated freeze-thaw cycles.
• Preservative: No Preservative
• Purity: Whole Antiserum
• Reconstitution Instructions: Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Alternate Names for EAAT2/GLT1 Antibody

• EAAT2
• GLT1
• member 2
• SLC1A2
• solute carrier family 1 (glial high affinity glutamate transporter), member 2
• Solute carrier family 1 member 2

Background

Mediates dihydrokainate-sensitive glutamate transport; may play a role in presynaptic terminal glutamate transport and synaptic transmission.; FUNCTION: Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Localized in brain and is highly enriched in the Purkinje cell layer in cerebellum, retina.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC1A2