E6AP/UBE3A Antibody (1077332) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clone: 1077332
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

E6AP/UBE3A Antibody (1077332) [Alexa Fluor® 594] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A
  Met1-Leu875
  Accession # Q05086
• Specificity: Detects human E6AP/UBE3A in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E6AP/UBE3A Antibody (1077332) [Alexa Fluor® 594]

• ANCR
• ANCREPVE6AP
• AS
• CTCL tumor antigen se37-2
• E6AP ubiquitin-protein ligase
• E6AP
• E6-AP
• EC 6.3.2.-
• EPVE6AP
• FLJ26981
• HPVE6A
• human papilloma virus E6-associated protein
• Human papillomavirus E6-associated protein
• Oncogenic protein-associated protein E6-AP
• Renal carcinoma antigen NY-REN-54
• UBE3A
• ubiquitin protein ligase E3A
• ubiquitin-protein ligase E3A

Background

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q05086(Human)