E6AP/UBE3A Antibody (1077332) [Alexa Fluor™ Plus 594]

• Clone: 1077332
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

E6AP/UBE3A Antibody (1077332) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human E6AP/UBE3A in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

1. Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
2. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
3. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
4. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
5. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
6. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.