E4F1 Antibody (4B10) - Azide and BSA Free

Western Blot: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10. Lane 1: E4F1 transfected lysatE (83.5 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: E4F1 Antibody (4B10) [H00001877-M03] - Analysis of monoclonal antibody to E4F1 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: E4F1 Antibody (4B10) [H00001877-M03] - Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4B10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

E4F1 Antibody (4B10) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GHQIIVQNVTMDEETALGPEAAAADTITIATPESLTEQVAMTLASAISEGTVLAARAGTSGTEQATVTMVSSEDIEILEHAGELVIASPEGQLEVQTVIV
• Specificity: E4F1 - E4F transcription factor 1 (4B10)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: E4F1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for E4F1 Antibody (4B10) - Azide and BSA Free

• E4F transcription factor 1p50E4F
• E4FMGC99614
• EC 6.3.2.-
• p120E4F
• Putative E3 ubiquitin-protein ligase E4F1
• Transcription factor E4F
• transcription factor E4F1

Background

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E4F1 Antibody (H00001877-M03)(1)

Publication using H00001877-M03

• Tsung-Hua H, Chia-Yi H, Chia-Wei W et al. Vorinostat decrease M2 macrophage polarization through ARID1A6488delG/HDAC6/IL-10 signaling pathway in endometriosis-associated ovarian carcinoma. Biomed Pharmacother. 2023-03-21 [PMID: 36958195]

Bioinformatics

• Gene Symbol: E4F1
• Entrez:
  • 1877(Human)
• Uniprot:
  • NP_004415(Human)