E2F-1 Antibody (2E10.)

Images

 
Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between CDK7 and E2F1. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-E2F1 ...read more
Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between CDK7 and E2F1. Huh7 cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-E2F1 ...read more
Proximity Ligation Assay: E2F-1 Antibody (2E10) [H00001869-M01] - Analysis of protein-protein interactions between MYBL2 and E2F1. Mahlavu cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and ...read more
ELISA: E2F-1 Antibody (2E10.) [H00001869-M01] - E2F-1 Antibody (2E10) [H00001869-M01] - Detection limit for recombinant GST tagged E2F1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, PLA
Clone
2E10.
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

E2F-1 Antibody (2E10.) Summary

Immunogen
E2F1 (NP_005216.1 348 a.a. - 437 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF
Specificity
E2F1 - E2F transcription factor 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
E2F1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for E2F-1 Antibody (2E10.)

  • E2F transcription factor 1
  • E2F1
  • E2F-1
  • PBR3
  • PRB-binding protein E2F-1
  • RBAP1
  • RBAP-1
  • RBBP3
  • RBBP-3
  • RBP3
  • Retinoblastoma-associated protein 1
  • Retinoblastoma-binding protein 3
  • transcription factor E2F1

Background

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol E2F1
Entrez
OMIM
Uniprot