Rat E-Selectin/CD62E ELISA Kit (Colorimetric) Summary
Specificity |
Recognizes both recombinant and natural Rat E-Selectin/CD62E. |
Standard Curve Range |
23.44-1500 pg/ml (example only; lot dependent) |
Sensitivity |
3.78 pg/ml |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 5.6 % |
Intra-Assay |
CV% < 3.1 |
Spike Recovery |
80-88% |
Sample Volume |
100 ul |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
SELE |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Kit Components
Components
|
- 1. 96 well microplate coated with Capture Antibody
- 2. Detection Antibody conjugated to HRP
- 3. Standards
- 4. Wash Buffer Concentrate
- 5. Dilution Buffer Concentrate
- 6. Color Reagent A
- 7. Color Reagent B
- 8. Stop Solution
|
Alternate Names for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)
Background
CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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