Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

Sandwich ELISA: Rat E-Selectin/CD62E ELISA Kit (Colorimetric) [NBP2-80383] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

• Reactivity: Rt
• Applications: ELISA
• Conjugate: HRP
• Suitable Sample Type: Serum
• Standard Curve Range: 23.44-1500 pg/ml (example only; lot dependent)
• Sensitivity: 3.78 pg/ml

Rat E-Selectin/CD62E ELISA Kit (Colorimetric) Summary

• Specificity: Recognizes both recombinant and natural Rat E-Selectin/CD62E.
• Standard Curve Range: 23.44-1500 pg/ml (example only; lot dependent)
• Sensitivity: 3.78 pg/ml
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.6 %
• Intra-Assay: CV% < 3.1
• Spike Recovery: 80-88%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SELE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage is content dependent.

Alternate Names for Rat E-Selectin/CD62E ELISA Kit (Colorimetric)

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for E-Selectin/CD62E ELISA Kit (NBP2-80383). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SELE