Monkey E-Selectin/CD62E ELISA Kit (Colorimetric)

ELISA: Primate E-Selectin/CD62E ELISA Kit (Colorimetric) [NBP1-92718]

Product Discontinued

Monkey E-Selectin/CD62E ELISA Kit (Colorimetric) Summary

• Specificity: This antibody detects the soluble fraction. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey E-selectin positive serum. There was no crossreactivity detected.
• Standard Curve Range: 1.9 - 60 U/mL
• Sensitivity: 0.4 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Spike Recovery: The amount of endogenous monkey E-selectin in unspiked serum was subtracted from the spike values. The overall mean recovery was 85%.
• Sample Volume: 20 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SELE

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Baboon

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey E-Selectin/CD62E ELISA Kit (Colorimetric)

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

Endothelial Leukocyte Adhesion Molecule-1 belongs to the selectin family of adhesion molecules. Together with L-selectin and P-selectin, E-selectin mediates the initial interactions of leukocytes and platelets with endothelial cells. Molecular structure: The extracellular part of all selectins consists of an aminoterminal c-type lectin domain which specifically binds to carbohydrate ligands. This is followed by an EGF-like domain, and, in the case of E-selectin, by 6 short consensus repeats. The transmembrane portion of the molecule is followed by a short cytoplasmic tail. Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. Together with P-selectin, E-selectin is expressed on cytokine-activated endothelial cells, and contributes to the adhesion of still resting leukocytes to the endothelium. This initial binding event is an essential prerequisite for the activation of the immune cells via different inflammatory mediators. In contrast to P-selectin, E-selectin is maximally expressed 2-4 hours after cell activation. Within the next 24-48 hours E-selectin is again eliminated from the cytoplasmic membrane by shedding into the circulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for E-Selectin/CD62E ELISA Kit (NBP1-92718). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: SELE