E-Selectin/CD62E Antibody [Unconjugated]

Images

 
E-Selectin/CD62E was detected in acetone fixed cryosections of human kidney tissue using Goat Anti-Human E-Selectin/CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 ° C. Tissues were stained with rabbit ...read more
E‑Selectin/CD62E was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human E‑Selectin/CD62E Polyclonal Antibody (Catalog # BBA18) overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

E-Selectin/CD62E Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human E-Selectin/CD62E
Specificity
This antibody has been screened using CHO cells transfected with cDNAs for E-Selectin, P-Selectin, L-Selectin, ICAM-1 and VCAM‑1. This antibody has shown to be only reactive with E-Selectin.
Source
N/A
Isotype
Serum
Clonality
Polyclonal
Host
Goat
Gene
SELE
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution
  • Immunohistochemistry 1:100 dilution
Reviewed Applications
Read 1 Review rated 5
using
BBA18 in the following applications:

Publications
Read Publications using
BBA18 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Serum.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute in 0.5 mL of sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody [Unconjugated]

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E-Selectin on healthy and diseased tissue. The human and mouse
E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for E-Selectin/CD62E Antibody (BBA18)(6)

We have publications tested in 2 confirmed species: Human, Rabbit.

We have publications tested in 3 applications: IHC-P, Neutralization, Western Blot.


Filter By Application
IHC-P
(2)
Neutralization
(1)
Western Blot
(3)
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Human
(5)
Rabbit
(1)
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Showing Publications 1 - 6 of 6.
Publications using BBA18 Applications Species
Kim J, Yoon Y, Lee J, Choi E, Yi N, Park K, Park J, Lotvall J, Kim Y, Gho Y Outer membrane vesicles derived from Escherichia coli up-regulate expression of endothelial cell adhesion molecules in vitro and in vivo. PLoS ONE, 2013;8(3):e59276. 2013 [PMID: 23516621] (Western Blot, Human) Western Blot Human
Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes. J Biol Chem, 2012;287(32):26478-94. 2012 [PMID: 22707717] (Western Blot, Human) Western Blot Human
Fenzi F, Rossi F, Rava M, Cavallaro T, Ferrari S, Rizzuto N Endothelial adhesion molecule expression is unaltered in the peripheral nerve from patients with AIDS and distal sensory polyneuropathy. J. Neuroimmunol., 2006;178(1):111-6. 2006 [PMID: 16859757] (IHC-P, Human) IHC-P Human
Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. Infect. Immun., 2005;73(7):3896-902. 2005 [PMID: 15972475] (IHC-P, Human) IHC-P Human
Miller AM, McPhaden AR, Preston A, Wadsworth RM, Wainwright CL TNFalpha increases the inflammatory response to vascular balloon injury without accelerating neointimal formation. Atherosclerosis, 2005;179(1):51-9. 2005 [PMID: 15721009] (Neutralization, Rabbit) Neutralization Rabbit
Tilghman RW, 2019, Hoover RL E-selectin and ICAM-1 are incorporated into detergent-insoluble membrane domains following clustering in endothelial cells. eaav5562, 2002;525(1):83-7. 2002 [PMID: 12163166] (Western Blot, Human) Western Blot Human

Review for E-Selectin/CD62E Antibody (BBA18) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using BBA18:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
Flow Other 01/16/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID: 22128816
SpeciesOther

Blocking

Blocking DetailsSee PMID: 22128816

Details

Detection NotesSee PMID: 22128816

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 22128816

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Recent Reviews

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Anonymous
01/16/2015
Application: Flow
Species: Other

Bioinformatics

Gene Symbol SELE