E-Selectin/CD62E Antibody [Unconjugated]

E-Selectin/CD62E was detected in perfusion fixed frozen sections of mouse thymus using Mouse E-Selectin/CD62E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF575) at 1.7 µg/mL overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Concentration: LYOPH

E-Selectin/CD62E Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin/CD62E
  Trp22-Pro557
  Accession # Q3U5F6
• Specificity: Detects mouse E-Selectin/CD62E in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human E-Selectin and recombinant rat E-Selectin is observed.
• Source: N/A
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: SELE

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for E-Selectin/CD62E Antibody [Unconjugated]

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E‑Selectin on healthy and diseased tissue. The human and mouse
E‑Selectin proteins share 81% amino acid similarity.

E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for E-Selectin/CD62E Antibody (AF575)(5)

Publications using AF575

• Mathias D, Mitchel R, Barclay M, Wyatt H, Bugden M, Priest N, Whitman S, Scholz M, Hildebrandt G, Kamprad M, Glasow A Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice. PLoS ONE, 2015;10(3):e0119661. 2015 [PMID: 25799423] (IHC, Mouse)
• Kato N, Yuzawa Y, Kosugi T The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J. Am. Soc. Nephrol., 2009;20(7):1565-76. 2009 [PMID: 19443639] (IHC-Fr, Mouse)
• Leeper NJ, Tedesco MM, Kojima Y, Schultz GM, Kundu RK, Ashley EA, Tsao PS, Dalman RL, Quertermous T Apelin prevents aortic aneurysm formation by inhibiting macrophage inflammation. Am. J. Physiol. Heart Circ. Physiol., 2009;296(5):H1329-35. 2009 [PMID: 19304942] (IHC-P, Mouse)
• Roelofs JJ, Teske GJ, Bonta PI, de Vries CJ, Meijers JC, Weening JJ, van der Poll T, Florquin S Plasminogen activator inhibitor-1 regulates neutrophil influx during acute pyelonephritis. Kidney Int., 2009;75(1):52-9. 2009 [PMID: 18800031] (IHC-P, Mouse)
• Scamuffa N, Siegfried G, Bontemps Y, Ma L, Basak A, Cherel G, Calvo F, Seidah NG, Khatib AM Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells. J. Clin. Invest., 2008;118(1):352-63. 2008 [PMID: 18064302] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: SELE
• Uniprot:
  • Q3U5F6()